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. Author manuscript; available in PMC: 2019 Jul 29.
Published in final edited form as: Cell Rep. 2019 Apr 9;27(2):400–415.e5. doi: 10.1016/j.celrep.2019.03.057

Figure 5. Histone Methylation and Acetylation Are Broadly Preserved on Mitotic Chromatin.

Figure 5.

(A) Changes in abundance of histone PTM categories between interphase and mitosis. (B) Changes in individual histone PTM abundance between interphase and mitosis. (C) Changes in abundance of particular BRD4-binding histone PTMs between interphase and mitosis. (D) Quantitative western blots of BRD4, GAPDH, and multiple histone PTMs during interphase, mitosis, or mitosis after 1 h of 250-nM JQ1 treatment. Non-JQ1 conditions were treated with 1 h of 250-nM DMSO. Results were normalized to within-lane background, GAPDH, and the interphase condition. Conditions with error bars represent n = 2 experiments, means ± SEM; other conditions are n = 1. (E) Changes in abundance of particular histone di-PTM combinations between interphase and mitosis. For (A)–(C) and (E), PTM abundance was normalized to peptide abundance.