Skip to main content
. 2019 Jul 29;19:170. doi: 10.1186/s12866-019-1525-4

Table 3.

Primer sequences for real-time PCR

Gene Primer sequence NCBI Reference Sequence: References
NF-κB p65

Forward 5′ - GGACAGCACCACCTACGATG - 3′

Reverse 5′ - CTGGATCACTTCAATGGCCTC - 3’

NM_009045.4 Present study
I-κB

Forward 5’ - CAGGAGCCAAAACCGACAAC - 3′

Reverse 5′ - TGGTTGTCAGGTCTGCAATTTT - 3’

NM_001306222.1 Present study
Fas

Forward 5’ - CCAAACGGAAATTGCAGGGG - 3′

Reverse 5′ - AAGCACCAGTTCACAGATGGA - 3’

NM_001146708.1 Present study
FADD

Forward 5’ - TGCTCCACCTATCCACCAGA - 3′

Reverse 5′ - CAATGCGGAAGGCGATTGAG - 3’

NM_010175.6 Present study
TRADD

Forward 5’ - GAGCTGCTGGAGTGCAACTA - 3′

Reverse 5′ - GGTCCGGGTACTTAGAGGGT - 3’

NM_001033161.2 Present study
Caspase-8

Forward 5’ - CCAGACAGAGAAGGGGCTTG - 3′

Reverse 5′ - TCACTGCCCAGTTCTTCAGC - 3’

NM_001080126.1 Present study
IL-1β

Forward 5’ - TCGTGCTGTCGGACCCATAT - 3′

Reverse 5′ - GTCGTTGCTTGGTTCTCCTTGT - 3’

NM_008361.4 Present study
IL-6

Forward 5’ - GACAAAGCCAGAGTCCTTCAGA - 3′

Reverse 5′ - TGTGACTCCAGCTTATCTCTTGG - 3’

NM_001314054.1 Present study
TNF-α

Forward 5’ - GCGGAGTCCGGGCAGGTCTA - 3′

Reverse 5′ - GGGGGCTGGCTCTGTGAGGA - 3’

NM_001278601.1 Present study
β-actin

Forward 5’ - TGCTGTCCCTGTATGCCTCTG - 3′

Reverse 5′ - TTGATGTCACGCACGATTTCC - 3’

NM_007393.4 Present study