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. 2019 Jul 29;17:86. doi: 10.1186/s12964-019-0393-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Nedd8 facilitated ubiquitination of HDAC1. a HL-60 cells transfected with constructs encoding HDAC1, His-ubiquitin (His-Ub), Flag-NUB1 (NEDD8 ultimate buster 1), and Myc-Mdm2 following incubation with MG132, a protease inhibitor. Ni2⁺ pull-down assay and Western blot analysis of (Ub)n-HDAC1 and Flag-NUB1 expression. b The Ni2⁺ pull down assay was evaluated by Western blot with appropriate antibodies in HL-60 cells expressing HDAC1, Flag-NUB1, His-ubiquitin, Myc-Mdm2, and NEDP1