Table 1.
Primer sequences used for real-time quantitative PCR
| Gene | Primer | Product size (bp) | GenBank accession No. |
|---|---|---|---|
| CSN1S1 |
F: CTTTTCAGACAATTCTACCAGCT R: AATTCACTTGACTCCTCACCAC |
171 | NM_181029.2 |
| CSN2 |
F: AGTCCAAAGTCCTGCCTGTTCC R: TGCCATATTTCCAGTCGCAGTC |
193 | XM_015471671.2 |
| CSN3 |
F: CAATACGCTGTGAGAAAGATGA R: AACTGGTTTCTGTTGGTAGTAA |
122 | NM_174294.2 |
| STAT5A |
F: CATGTCCCTCAAGAGGATCA R: TCATTGCTGCCAACACTG |
108 | NM_001012673.1 |
| ACACA |
F: GATCCAGGCCATGCTAAG R: CTGTTTCTCCAGCCACTC |
103 | XM_024979607.1 |
| FASN |
F: AGGACCTCGTGAAGGCTGTGA R: CCAAGGTCTGAAAGCGAGCTG |
85 | XM_005220997.3 |
| S6K1 |
F: GGACATGGCAGGGGTGTTT R: GGTATTTGCTCCTGTTACTTTTCG |
283 | NM_205816.1 |
| ACSL1 |
F: GCCGCATTTCACTTTTACTGC R: AGCTCTTTAGGGCAAACCCC |
136 | NM_001076085.1 |
| ACSS2 |
F: GGGCGAATGCCTCTACTGC R: GCTGGGTGATGATGGATGG |
254 | NM_001105339.1 |
| GAPDH |
F: GGGTCATCATCTCTGCACCT R: GGTCATAAGTCCCTCCACGA |
177 | NM_001034034.2 |
CSN1S1: casein alpha s1; CSN2: casein beta; CSN3: casein kappa; STAT5A: signal transducer and activator of transcription 5A; ACACA: acetyl-CoA carboxylase 1; FASN: fatty acid synthase; S6K1: ribosomal protein S6 kinase 1; ACSL1: acyl-CoA synthetase long-chain family member 1; ACSS2: acyl-CoA synthetase short-chain family member 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase