TABLE 1.

Unbiased proteomics analysis of myotubes treated with AmAc for 24 h compared to untreated cells^a

NCBI accession no.	Protein	Designation	Mol wt (kDa)	LFQ intensity at 24 h				LFQ intensity for Ct				Ratio of 24-h/Ct LFQ intensity	P value by t test	MS/MS count
				Expt 1	Expt 2	Expt 3	Avg	Expt 1	Expt 2	Expt 3	Avg
P60867	40S ribosomal protein S20	Rps20	13.373	1.81E+09	1.91E+09	1.87E+09	1.86E+09	2.07E+09	1.97E+09	2.04E+09	2.03E+09	0.92	0.018	152
P63325	40S ribosomal protein S10	Rps10	18.916	4.04E+08	4.42E+08	4.33E+08	4.26E+08	4.64E+08	5.07E+08	4.97E+08	4.89E+08	0.87	0.022	117
P62242	40S ribosomal protein S8	Rps8	24.205	2.17E+09	2.73E+09	2.46E+09	2.46E+09	2.85E+09	3.19E+09	3.01E+09	3.02E+09	0.81	0.040	300
P62754	40S ribosomal protein S6	Rps6	28.68	1.55E+09	1.90E+09	1.75E+09	1.74E+09	2.35E+09	2.29E+09	2.34E+09	2.32E+09	0.75	0.005	251
P62267	40S ribosomal protein S23	Rps23	15.807	5.13E+08	6.54E+08	5.60E+08	5.76E+08	7.20E+08	7.31E+08	9.23E+08	7.92E+08	0.73	0.050	133
P62281	40S ribosomal protein S11	Rps11	18.431	1.24E+09	1.37E+09	1.27E+09	1.29E+09	1.82E+09	1.49E+09	1.41E+09	1.57E+09	0.82	0.100	198
P62855	40S ribosomal protein S26	Rps26	13.015	6.72E+08	7.12E+08	6.49E+08	6.77E+08	6.86E+08	8.06E+08	7.41E+08	7.44E+08	0.91	0.164	78
P63276	40S ribosomal protein S17	Rps17	15.524	1.54E+09	1.58E+09	1.65E+09	1.59E+09	1.65E+09	1.63E+09	1.84E+09	1.71E+09	0.93	0.190	165
P25444	40S ribosomal protein S2	Rps2	31.231	1.58E+09	1.77E+09	1.69E+09	1.68E+09	1.90E+09	1.72E+09	1.75E+09	1.79E+09	0.94	0.219	232
P62264	40S ribosomal protein S14	Rps14	16.273	1.53E+09	1.65E+09	1.56E+09	1.58E+09	1.74E+09	1.53E+09	1.65E+09	1.64E+09	0.96	0.435	154
P62274	40S ribosomal protein S29	Rps29	6.6767	5.77E+08	6.43E+08	5.40E+08	5.87E+08	6.81E+08	6.02E+08	5.73E+08	6.19E+08	0.95	0.512	56
P62301	40S ribosomal protein S13	Rps13	17.222	5.37E+08	4.54E+08	4.94E+08	4.95E+08	6.56E+08	4.41E+08	4.94E+08	5.30E+08	0.93	0.634	120
P27659	60S ribosomal protein L3	Rpl3	46.109	2.41E+09	2.73E+09	2.56E+09	2.56E+09	3.05E+09	3.08E+09	3.09E+09	3.07E+09	0.83	0.005	377
Q9CPR4	60S ribosomal protein L17	Rpl17	21.423	1.37E+09	1.60E+09	1.36E+09	1.44E+09	1.88E+09	1.79E+09	1.70E+09	1.79E+09	0.81	0.023	225
P47963	60S ribosomal protein L13	Rpl13	24.305	1.84E+09	2.10E+09	1.96E+09	1.97E+09	2.44E+09	2.50E+09	2.69E+09	2.54E+09	0.77	0.006	229
Q9Z2Q5	39S ribosomal protein L40, mitb	Mrpl40	24.301	5.55E+07	4.90E+07	4.49E+07	4.98E+07	6.26E+07	7.00E+07	6.07E+07	6.45E+07	0.77	0.025	46
P41105	60S ribosomal protein L28	Rpl28	15.733	5.35E+08	6.39E+08	5.51E+08	5.75E+08	7.57E+08	7.45E+08	7.53E+08	7.52E+08	0.77	0.006	156
P61255	60S ribosomal protein L26	Rpl26	17.258	5.93E+08	7.08E+08	6.82E+08	6.61E+08	9.70E+08	9.00E+08	8.02E+08	8.91E+08	0.74	0.019	141
P62717	60S ribosomal protein L18a	Rpl18a	20.732	2.82E+08	3.72E+08	3.69E+08	3.41E+08	4.67E+08	4.90E+08	5.20E+08	4.92E+08	0.69	0.010	108
P12970	60S ribosomal protein L7a	Rpl7a	29.976	6.47E+08	6.23E+08	6.11E+08	6.27E+08	9.71E+08	9.43E+08	8.53E+08	9.22E+08	0.68	0.001	191
P47911	60S ribosomal protein L6	Rpl6	33.509	1.35E+09	1.65E+09	1.53E+09	1.51E+09	2.33E+09	2.27E+09	2.14E+09	2.25E+09	0.67	0.002	246
Q6ZWV7	60S ribosomal protein L35	Rpl35	14.552	3.94E+08	5.16E+08	4.34E+08	4.48E+08	6.72E+08	7.17E+08	6.46E+08	6.78E+08	0.66	0.005	73
P62918	60S ribosomal protein L8	Rpl8	28.024	9.82E+08	1.28E+09	1.12E+09	1.13E+09	1.82E+09	1.59E+09	1.78E+09	1.73E+09	0.65	0.006	205
Q8BP67	60S ribosomal protein L24	Rpl24	17.779	3.58E+08	4.28E+08	3.97E+08	3.94E+08	6.99E+08	6.28E+08	6.35E+08	6.54E+08	0.60	0.001	110
Q9D1R9	60S ribosomal protein L34	Rpl34	13.293	1.38E+08	1.62E+08	1.75E+08	1.58E+08	3.30E+08	2.45E+08	2.56E+08	2.77E+08	0.57	0.014	48
Q9CR57	60S ribosomal protein L14	Rpl14	23.564	1.84E+08	1.79E+08	1.99E+08	1.87E+08	3.77E+08	3.54E+08	2.95E+08	3.42E+08	0.55	0.004	75
P35980	60S ribosomal protein L18	Rpl18	21.644	1.65E+08	2.64E+08	1.81E+08	2.03E+08	3.06E+08	4.06E+08	5.40E+08	4.17E+08	0.49	0.045	92
P14148	60S ribosomal protein L7	Rpl7	31.419	3.59E+08	5.29E+08	3.65E+08	4.18E+08	7.76E+08	8.50E+08	9.51E+08	8.59E+08	0.49	0.004	161
P47964	60S ribosomal protein L36	Rpl36	12.215	3.93E+08	4.58E+08	4.83E+08	4.45E+08	5.49E+08	5.12E+08	6.25E+08	5.62E+08	0.79	0.052	93
P19253	60S ribosomal protein L13a	Rpl13a	23.464	5.08E+08	7.59E+08	5.99E+08	6.22E+08	7.35E+08	7.91E+08	7.84E+08	7.70E+08	0.81	0.121	157
P62892	60S ribosomal protein L39	Rpl39	6.4066	3.29E+08	3.83E+08	3.53E+08	3.55E+08	3.75E+08	4.06E+08	4.84E+08	4.22E+08	0.84	0.138	55
Q9D823	60S ribosomal protein L37	Rpl37	11.078	500,000	1,372,000	3,744,100	1.87E+06	2,985,600	2.45E+07	500,000	9.33E+06	0.20	0.387	7

^aLFQ, label-free quantitation; Ct, control group.

^bmit, mitochondrial.