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. 2019 Jul 29;39(16):e00024-19. doi: 10.1128/MCB.00024-19

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Copyright © 2019 Tamura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 9 — Electron microscopic analyses for autophagosomal structures and WIPI2 localization in FIP200^−/− or Atg13^−/− MEFs under hyperosmotic stress. (A) FIP200^−/− MEFs were cultured in DMEM with or without (control) 0.8 M sucrose for 1 h and then fixed for EM. The boxed region in the middle is shown enlarged on the right. (B) FIP200^−/− MEFs and Atg13^−/− MEFs were cultured with 0.8 M sucrose for 30 min and then fixed for immuno-EM using anti-WIPI2 antibody. Note that small autophagosome/IM-like profiles with double membranes (panel A, right, arrowheads), and those labeled with silver-enhanced particles indicating WIPI2 were detected. Asterisk, large endosome-like structures; L, lysosome-like profiles; m, mitochondria; M, degenerated mitochondria; N, nucleus. Bars, 0.5 μm.