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. 2019 Jul 29;39(16):e00035-19. doi: 10.1128/MCB.00035-19

FIG 5.

FIG 5

Splicing inhibition increases intron retention and reduces mature mRNA formation. (A) Relative mature IL-2 level in EL4 cells after 6 h of PMA stimulation in the presence or absence of Pld B in the last 2 h (i.e., 4 to 6 h) after PMA stimulation (mean ± SD; n = 6); (B) relative level of IL-2 intron-retained transcripts under the same experimental conditions described for panel A (mean ± SD; n = 3); (C) relative mature Tnfsf11, IL-4a, and CPEB4 levels in EL4 cells after 6 h of PMA stimulation in the presence or absence of Pld B in the last 2 h (i.e., 4 to 6 h) after PMA stimulation (mean ± SD; n = 3). ***, P < 0.0001; **, P < 0.001; *, P < 0.01 (Student's unpaired t test, done using GraphPad software).