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. 2019 Jul 24;10:789. doi: 10.3389/fphar.2019.00789

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Copyright © 2019 Liu, Liu, He, Mu, Di, Shen, Liu, Gao, Wang, Chen, Fang, Li and Tian

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Lir on the ERS-related pathway induced by H/R injury in HUVECs. (A) mRNA expression levels of ERS markers were determined by RT-PCR. (B) Protein expression levels of ERS markers were determined by Western blot. mRNA expression levels of ERS marker proteins GRP78 (C) and ATF4 (D). Protein expression levels of ERS marker proteins GRP78 (E), p-PERK (F), and ATF4 (G). Expression of β-actin was used for the loading control. Results were normalized to the percentage of β-actin expression. Data are mean ± SE. *P < 0.05, **P < 0.01 compared to Sham group; ^#P < 0.05, ^##P < 0.01 compared to H/R group.