Table 2.
Five-level and three-variable central composite circumscribed (CCC) design for total flavonoids (TF) extraction in D. indicum var. aromaticum and observed responses.
| Run | X 1 (min) | X 2 (g/mL) | X 3 (°C) | TF extraction rate (g QEb/100 g DW) |
|---|---|---|---|---|
| 1 | 20 (−1) | 1 : 10 (−1) | 40 (−1) | 0.2586 |
| 2 | 40 (1) | 1 : 10 (−1) | 40 (−1) | 0.3457 |
| 3 | 20 (−1) | 1 : 30 (1) | 40 (−1) | 0.2385 |
| 4 | 40 (1) | 1 : 30 (1) | 40 (−1) | 0.3104 |
| 5 | 20 (−1) | 1 : 10 (−1) | 60 (1) | 0.4192 |
| 6 | 40 (1) | 1 : 10 (−1) | 60 (1) | 0.4452 |
| 7 | 20 (−1) | 1 : 30 (1) | 60 (1) | 0.3675 |
| 8 | 40 (1) | 1 : 30 (1) | 60 (1) | 0.4109 |
| 9 | 13 (−1.68) | 1 : 20 (0) | 50 (0) | 0.3177 |
| 10 | 47 (1.68) | 1 : 20 (0) | 50 (0) | 0.4330 |
| 11 | 30 (0) | 1 : 03 (−1.68) | 50 (0) | 0.2115 |
| 12 | 30 (0) | 1 : 37 (1.68) | 50 (0) | 0.4189 |
| 13 | 30 (0) | 1 : 20 (0) | 33 (−1.68) | 0.2644 |
| 14 | 30 (0) | 1 : 20 (0) | 67 (1.68) | 0.4307 |
| 15 | 30 (0) | 1 : 20 (0) | 50 (0) | 0.3811 |
| 16 | 30 (0) | 1 : 20 (0) | 50 (0) | 0.3822 |
| 17 | 30 (0) | 1 : 20 (0) | 50 (0) | 0.3840 |
| 18 | 30 (0) | 1 : 20 (0) | 50 (0) | 0.4037 |
| 19 | 30 (0) | 1 : 20 (0) | 50 (0) | 0.3788 |
| 20 | 30 (0) | 1 : 20 (0) | 50 (0) | 0.3321 |
Note. Numbers in brackets are coded independent variables. bQuercetin equivalent.