Table 2.

RT-PCR and RT-qPCR primers used in this study

Gene	Forward (5′–3′)	Reverse (5′–3′)	Annealing temp. (°C)
Primers used for RT-PCR
OCT4	GTGGAGGAAGCTGACAACAA	CAGGTTTTCTTTCCCTACGCT	54
NANOG	AGCTACAGCATGATGCAGGA	GGTCATGGAGTTGTACTGCA	54
SOX2	TCCAACATCCTGAACCTCAG	GACTGGATGTTCTGGGTCTG	55
ALBUMIN	CCTTTGGCACAATGAAGTGGGTAACC	CAGCAGTCAGCCATTCACCATAGG	58
A1AT	CCAACAGCACCAATATCCATCTTC	GTCCTCTTCCTCGGTGTCCTTG	60
HNF1α	AGACTTCACGCCACCCATCCTC	TTCCTCCGCCCCTTCTTGGTTG	60
ASGR	TAGGAGCCAAGCTGGAGAAA	ACCTGCAGGCAGAAGTCATC	56
ACTIN	GCACTCTTCCAGCCTTCCTTCC	CTGCTGTCACCTTCACCGTTCC	60
FOXA2	GCGACCCCAAGACCTACAG	GGTTCTGCCGGTAGAAGGG	60
GATA4	CTAGACCGTGGGTTTTGCAT	TGGGTTAAGTGCCCCTGTAG	56
HNF4α	CGGGTGTCCATACGCATCCTTG	GACCCTCCCAGCAGCATCTCCT	60
Primers used for RT-qPCR
CYP2D6	AAGTACAGGGCTTCCGCATCC	GGGCTCACCAGGAAAGCAA	60
CYP3A4	AGAAAGTCGCCTCGAAGATACAC	CAGAGCTTTGTGGGCTCAGTT	56
CYP2A6	GCCCGCAGAGTCAAAAAGGA	CCCCTTCTCATTCAGGAAGTGCT	57
SREBP2	CTCCATTGACTCTGAGCCAGGA	GAATCCGTGAGCGGTCTACCAT	60
HMGCR	CACGATGCATAGCCATCCTG	GTGCTTGCTCTGGAAAGGTC	64
PCSK9	TGTATGGTCAGCACACTCGG	TAGACACCCTCACCCCCAAA	62
APOA1	AGGCTCGGCATTTCTGGCAG	CGCTGTCCCAGTTGTCAAGG	59
APOB	AGCGTTCACCGATCTCCATCTG	TCAGATTCCCGGACC CTCAACT	59
APOC3	GTTACATGAAGCACGCCACC	CTCGCAGGATGGATAGGCAG	59
APOE	TGGGTCGCTTTTGGGATTAC	AGTTGTTCCTCCAGTTCCGA	59