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. 2019 Jul 29;10:218. doi: 10.1186/s13287-019-1334-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Induction of γH2AX- and 53BP1-foci formation after exposure to irradiation in aging MSCs in vitro. a MSCs were cultured for 1, 4, and 8 weeks in vitro. Repair foci formation is shown in MSCs 90 min after sham-irradiation (0 Gy) or after 2 Gy of γ-irradiation by immunofluorescence staining for γH2AX (red) and 53BP1 (green). Nuclear counterstaining was done using DAPI. b Quantification γH2AX- and 53BP1-foci formation in MSCs was done 90 min after sham-irradiation (0 Gy) or after γ-irradiation with 0.05, 0.5, 2, and 6 Gy using the Keyence BZ-II Analyzer software (see Additional file 6: Figure S1). Mean number of foci ± SEM (n = 3) are calculated from at least 50 analyzed nuclei