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. Author manuscript; available in PMC: 2019 Jul 30.
Published in final edited form as: Methods Mol Biol. 2018;1764:101–121. doi: 10.1007/978-1-4939-7759-8_7

Fig. 4.

Fig. 4

PCR steps performed for deep sequencing preparation of SSM libraries. Sequential PCR reactions to amplify the genes of interest and attach the Illumina adapters are shown for SSM library 2 (gold). (a) After extracting the plasmid DNA from yeast cells, SSM libraries are amplified by PCR using a set of inner primers containing a segment that overlaps with the gene of interest (light blue) and the Illumina universal sequence (purple). (b) A second round of PCR is performed to attach the Illumina adapter sequence using a set of outer primers which contain an overlapping region to the Illumina universal sequence (purple), a unique barcode on the reverse primer (green), and Illumina adapter sequences (yellow)