Figure S1.

Values used by Compusyn to calculate synergy between Osim and CQ. Fa was calculated for Osim (2.5, 4, and 6.5 µM) and CQ (10, 30, and 75 µM) alone and combination. For each drug combination DRI was calculated where DRI=1, >1, and <1 indicated no dose reduction, a favorable dose reduction, and no favorable dose reduction, respectively. Mean ± SEM was plotted using GraphPad Prism Version 5. Bars represent the mean on quadruplets observed data with SEM.

Abbreviations: Osim, osimertinib; CQ, chloroquine; Fa, Fractional inhibition; DRI, dose-reduction index; SEM, standard error of mean.