Figure 5.

Phosphorylated n-cofilin is localized to Map2-positive neuronal processes but not to Reelin-immunoreactive CR cells and Nestin-positive or RC2-positive radial glial fibers from E17.5 wild-type animals. Single confocal scans from z-series are shown with orthogonal views taken at the levels indicated by white dashed lines. Arrows label structures identifiable in the X, Y, and Z planes. A, Single confocal section double-immunostained for p-cofilin and Map2. Apical neuronal processes close to the marginal zone are colabeled. B, Single confocal section double-stained for p-cofilin and Reelin shows minimal colocalization. C, D, Virtually no colocalization of p-cofilin and the radial glial markers Nestin and RC2, respectively, is seen in single confocal sections. The yellow staining of some Nestin-positive processes is likely the result of intimate contact with strongly p-cofilin-positive neurons (white arrows). Scale bars, 50 μm.