Migration of human microglia, in vitro. A, Schematic diagram for the treatment of microglia with PBS, Aβ1–42, or Aβ42–1 and supernatant application, in the absence and presence of anti-Flt-1 Ab, in the transwell microglial migration assay. B, The bar graph shows the number of migrating microglia with the different treatments and the effects of anti-Flt-1 Ab on migration (n = 5 samples/group). *p < 0.05 for peptide versus PBS treatment; #p < 0.05 for effects of anti-Flt-1 Ab on migration with peptide application.