A, Immunoblot (IB) analysis of the expressions of pJNK, JNK, pPERK, PERK, GRP78, and peIF2α in hypothalamic protein extracts obtained from rats treated intracerebroventricularly with diluent (DL) or arachidic acid (C20:0) for 1–3 d; some rats were intracerebroventricularly cannulated but received no treatment (−). B, Immunoprecipitation (IP) analysis of the associations of TLR2 and TLR4 with MyD88 in hypothalamic protein extracts obtained from nonintracerebroventricular cannulated (CT) rats, and rats treated intracerebroventricularly with DL or C20:0 for 3 d. The depicted blots are representative of five distinct experiments.