A, Immunoblot (IB) analysis of expression of pJNK, JNK, pPERK, PERK, GRP78, and peIF2α in hypothalamic protein extracts obtained from rats not intracerebroventricularly cannulated (CT), and from rats treated intracerebroventricularly with diluent (DL), arachidic acid (C20:0), or PBA plus arachidic acid (PBA+C20:0) for 3 d. B, Immunoprecipitation (IP) analysis of the associations of TLR2 and TLR4 with MyD88 in hypothalamic protein extracts obtained from rats treated as described in A. The depicted blots are representative of five distinct experiments.