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. 2009 Mar 25;29(12):3808–3815. doi: 10.1523/JNEUROSCI.5301-08.2009

Figure 4.

Figure 4.

Organizational effects of gonadal steroids on ACh levels in female rats. At postnatal days 0 and 1, female pups were given oil (neo oil), testosterone (neo T), 17β-estradiol (neo E), or 5α-dihydrotestosterone (neo DHT). After maturation, the rats were bilaterally ovariectomized and given testosterone replacement. A, Neo oil or neo DHT rats exhibited relatively low ACh levels, whereas neo T or neo E rats enhanced the ACh levels compared with male rats. B, Overall levels of ACh and spontaneous locomotor activity were statistically summarized for neonatally steroid-treated female rats. Neo T or neo E treatment significantly enhanced the mean ACh levels in female rats without changing the spontaneous locomotor activity. C, In simple linear regression analysis, neo T or neo E data had significantly steeper slope than neo oil data. *p < 0.01 compared with neo oil. The number of rats in each group is shown in parentheses. Data are expressed as the mean ± SEM.