Figure 3.

RA-mediated growth cone turning requires protein synthesis. A, Intact neurites in the presence of anisomycin (4.5 × 10⁻⁵ m) failed to turn toward a gradient of RA. i, 0 min; ii, 33 min. Scale bar, 30 μm. iii, Histogram depicts maximum turning angle of intact growth cones to RA in the presence of the translational inhibitor, anisomycin, and each bar represents one growth cone. Positive values represent a turn toward the pipette and negative values a turn away from the pipette. B, Neurites isolated from their cell body also fail to turn toward the RA gradient when in the presence of anisomycin. i, 0 min; ii, 103 min. Scale bar, 30 μm. iii, Histogram depicts the maximal turning angle of isolated growth cones toward RA in the presence of the translational inhibitor, anisomycin. C, Summary graph showing the mean turning angles toward RA of intact and isolated growth cones in the presence of anisomycin. *p < 0.05 compared with CM.