Figure 4.

Anti-Ly6G/Gr-1 treatment alters cytokine and growth factor levels within the injured spinal cord at 48 h and 5 d after SCI. A, Quantification of inflammatory protein levels isolated from the injured spinal cord at 48 h after injury reveals a significant increase in MIP-1γ/CCL9, KC/CXCL1, G-CSF, and MCP-1/CCL2 protein levels in neutrophil-depleted animals (white bars) versus isotype (black bars) or sham controls (gray bars). Data are represented as mean protein levels [arbitrary units (A.U.)] ± SEM, n = 4 per group. ANOVA, Holm-Sidak method. *p < 0.05, isotype versus α-Ly6G/Gr-1-treated animals. ^#p < 0.05, sham versus isotype. ^†p < 0.05 sham versus α-Ly6G/Gr-1-treated animals. Analysis of growth factor mRNA levels isolated from the injured spinal cord at 48 h (B) and 5 d (C) after SCI using quantitative RT-PCR arrays. Plots of growth factor gene expression with a threefold change or higher are displayed, with α-Ly6G/Gr-1-treated (y-axis) and isotype controls (x-axis) in B and C. Upregulated genes are shown in red, whereas downregulated genes are shown in green. MCP-1/CCL2 monocyte chemoattractant protein-1, KC/CXCL1 keratinocyte derived chemokine, G-CSF granulocyte colony stimulating factor, MIP-1γ/CCL9, macrophage inflammatory protein-1 gamma, Bone morphogenic protein (BMP), fibroblast growth factor (FGF), Interleukin (IL), neutrotrophic factor (NTF), Transforming Growth factor (TGF), vasculature endothelial growth factor (VEGF).