NO donors increase the locomotor frequency. A, Locomotor activity was induced by NMDA (100 μm) and monitored by recording ventral roots on opposite sides in the same segment. B, Subsequent application of the NO donor DEA/NO (150 μm) increases the locomotor frequency. C, Averaged data from all the experiments showing the potentiating effect of DEA/NO on the locomotor frequency (p < 0.0001, n = 18). D, Averaged data from all the experiments showing the increase in the frequency induced by SNAP (p < 0.0001, n = 8).