Figure 6.

Genetic visualization of single mitral cells. B–F, Single-cell labeling was performed by injecting a mixture of two plasmids, plhx2a:GAL4-VP16-S and pUAS:gap-YFP (B₁–B₃, C–E), or ptbr2a:GAL4-VP16 and pUAS:gap-YFP (B₄, F), into 1–4 cell-stage embryos. The dendritic innervation pattern and the axon trajectory were examined at 7 dpf. A, Schematic representation of OB glomerular clusters and mG numbering. B₁–B₄, Typical dendritic morphologies of labeled MCs. Arrows and arrowheads denote the axons and the dendritic tufts, respectively. C₀–F₀, Examples of glomerular innervation patterns of four MC classes. C₁–C₃, Axon projection patterns of mG1–MCs in three different larvae. D₁–D₃, Axon projection patterns of mG6–MCs in three different larvae. E₁, E₂, Axon projection patterns of lG–MCs in two different larvae. F₁, An axon projection pattern of avG-MC in a larva. C₀–F₀, Frontal views with dorsal to the top. C₁–F₁, Dorsal views with anterior to the top. a, Anterior; p, posterior; d, dorsal; l, lateral. Scale bar: (in B₄) B₁–B₄, 10 μm; (in C₀) C₀–F₀, 20 μm; (in F₁), C₁–F₁, 50 μm.