Figure 7.

GABAergic signaling affects Ca²⁺ activity in astrocytes and migration of neuroblasts. A, Application of GABA_A receptor antagonist bicuculline (Bic, 100 μm) diminishes spontaneous Ca²⁺ fluctuations in the astrocytes. Time-lapse video imaging illustrating changes in the fluorescent intensity under control conditions (left panels) and after application of bicuculline (right panels) over time (indicated in seconds in the top right corner of each photograph). Scale bar, 10 μm. B, Individual experiments demonstrating that bath application of GABA (10 μm) after application of TrkB–Fc (1 μg/ml) as well as TrkB–Fc application after GABA application do not affect the average distance that migrating cells propagate. The time periods for TrkB–Fc and GABA applications are shown by black lines. Each time point represents the average value for 20–30 cells. C, Summary graphs illustrating that bath application of GABA (10 μm) after application of TrkB–Fc (1 μg/ml) and bath application of TrkB–Fc (1 μg/ml) after application of GABA (10 μm) do not affect the average distance that migrating cells propagate (per 15 s), the percentage of cells in the migratory phase, and the duration of stationary period.