Table 1.
Migration of neuronal precursors and Ca2+ fluctuations in astrocytes in the acute slices of the adult mouse forebrain
| Experiment | Distance of migration (μm) | Cells in the migratory phase (%) | Duration of the resting period (s) | Rate of migration (μm/h) | Frequency of Ca2+ fluctuations (peak/min) | Number of cells/slices/animals | |
|---|---|---|---|---|---|---|---|
| Migration in the RMS control | 0.32 ± 0.02 | 47.4 ± 2.0 | 492.3 ± 41.5 | 121.2 ± 3.9 | 64/14/10 | ||
| Application of BDNF (10 ng/ml) | Ctrl | 0.34 ± 0.01 | 44.9 ± 2.0 | 274.2 ± 13.8 | 136.4 ± 5.2 | 376/4/4 | |
| BDNF | 0.43 ± 0.02* | 57.4 ± 1.2* | 147.5 ± 5.6* | 142.6 ± 6.9 | |||
| Application of TrkB–Fc (1 μg/ml) | IgG–Fc | 0.33 ± 0.02 | 49.4 ± 2.1 | 260.5 ± 12.7 | 115.7 ± 7.3 | 162/4/4 | |
| TrkB–Fc | 0.16 ± 0.04* | 24.3 ± 6.2* | 713.2 ± 160.0* | 88.3 ± 8.8 | 194/4/4 | ||
| Application of p75NTR (20 μg/ml) | IgG–Fc | 0.33 ± 0.03 | 47.0 ± 3.3 | 243.6 ± 28.1 | 121.1 ± 6.7 | 91/4/4 | |
| p75NTR | 0.16 ± 0.01* | 24.6 ± 1.1* | 558.7 ± 2.9** | 101.5 ± 6.6 | 138/5/3 | ||
| Application of K252a (200 nm) | Ctrl | 0.33 ± 0.05 | 48.3 ± 7.9 | 293.5 ± 44.2 | 135.3 ± 4.1 | 125/2/2 | |
| K252a | 0.30 ± 0.06 | 44.4 ± 6.9 | 294.5 ± 55.9 | 127.1 ± 1.9 | |||
| p75NTR-WT | 0.33 ± 0.01 | 47.1 ± 1.4 | 259.4 ± 10.9 | 124.4 ± 4.3 | 150/5/4 | ||
| p75NTR-KO | 0.18 ± 0.03* | 26.9 ± 4.2** | 735.1 ± 101.1** | 121.7 ± 8.4 | 200/6/6 | ||
| Application of BDNF (10 ng/ml) in p75NTR-KO | Ctrl | 0.19 ± 0.06 | 25.2 ± 6.4 | 750.5 ± 158.4 | 121.7 ± 13.3 | 168/4/4 | |
| BDNF | 0.2 ± 0.06 | 26.2 ± 7.1 | 760.0 ± 156.3 | 125.9 ± 10.3 | |||
| Application of TrkB–Fc (1 μg/ml) in p75NTR-KO | Ctrl | 0.18 ± 0.06 | 30.1 ± 1.9 | 704.2 ± 38.4 | 121.6 ± 2.7 | 88/2/2 | |
| TrkB–Fc | 0.18 ± 0.02 | 28.8 ± 2.1 | 721.8 ± 21.1 | 122.4 ± 11.4 | |||
| Ca2+ activity in the RMS control | 0.49 ± 0.0004 | 125/27/10 | |||||
| Application of GABA (10 μm) | Ctrl | 0.41 ± 0.04 | 55.7 ± 2.9 | 262.0 ± 33.7 | 110.8 ± 10.6 | 0.42 ± 0.001 | 27/4/3 for Ca2+ activity |
| GABA | 0.17 ± 0.04** | 28.1 ± 5.1* | 584.6 ± 16.1** | 79.8 ± 7.9* | 0.58 ± 0.001** | 146/5/5 for migration | |
| Application of bicuculline methochloride (100 μm) | Ctrl | 0.24 ± 0.01 | 40.6 ± 1.8 | 255.0 ± 27.3 | 93.8 ± 3.9 | 0.52 ± 0.0007 | 53/7/5 for Ca2+ activity |
| Bic | 0.35 ± 0.03* | 51.5 ± 2.8* | 168.0 ± 17.7* | 135.3 ± 6.9* | 0.32 ± 0.001* | 130/7/5 for migration | |
| Application of TrkB–Fc (1 μg/ml)–TrkB–Fc (1 μg/ml) | TrkB–Fc | 0.19 ± 0.04 | 28.5 ± 5.8 | 814.0 ± 79.0 | 109.1 ± 2.5 | 67/2/2 | |
| Application of TrkB–Fc (1 μg/ml)–GABA (10 μm) | GABA | 0.16 ± 0.06 | 25.5 ± 9.0 | 864.5 ± 281.0 | 114.3 ± 4.5 | 105/2/2 | |
| Application of GABA (10 μm)–TrkB–Fc (1 μg/ml) | GABA | 0.14 ± 0.02 | 26.1 ± 4.7 | 704.6 ± 8.8 | 111.5 ± 11.6 | 71/2/2 | |
| TrkB–Fc | 0.11 ± 0.0001 | 19.9 ± 0.06 | 781.2 ± 9.8 | 109.1 ± 3.4 | |||
| Application of 2-APB (100 μm) | Ctrl | 0.32 ± 0.05 | 48.4 ± 0.3 | 276.5 ± 16.2 | 120.5 ± 2.8 | 0.46 ± 0.048 | 40/4/4 for Ca2+ activity |
| 2-APB | 0.01 ± 0.001** | 1.1 ± 1.1* | 738.0 ± 37.9 | 87.5 ± 0.3* | 0.08 ± 0.025*** | 45/2/2 for migration |
Bic, Bicuculline; Ctrl, control; KO, knock out; WT, wild type.