Figure 3.

Choline acetyltransferase and parvalbumin immunohistochemistry of the NBM. A–D, Panels (10× magnification) depict ChAT-immunoreactive cells (A, B) and parvalbumin-immunoreactive cells (C, D) in the NBM of a typical sham-lesioned (A, C) and NBM-lesioned (B, D) rat. A loss of ChAT-immunoreactive cells (B), but not parvalbumin-immunoreactive cells (D), is apparent in the NBM-lesioned rat. The rectangular outlines superimposed on the rat brain coronal schematics delineate the NBM cell-counting frames, and the solid gray fill illustrates the typical extent of ChAT-immunoreactive cell loss. Rat brain schematics were adapted from Paxinos and Watson (2007) and displayed coordinates refer to the anterior–posterior plane.