Table 1.

Oligonucleotide sequences used in nested-PCR to generate partial sequences for X. unicolor and P. regius LWS, SWS1, and Rh1 cone opsin genes and full-length sequences for in vitro regeneration of sunbeam LWS, SWS1, and Rh1 visual pigments

Primer	Sequence
AOASF1	5′-CGCGAGAGATACATNGTNRTNTGYAARCC-3′
AOASF2	5′-ATTTTAGAAGGTCTGCCRGWSNTCNTGYGG-3′
AOASR1	5′-ATTGGTCACCTCCTTYTCNGCYYTYTGNGT-3′
AOASR2	5′-CCCGGAAGACGTAGATGANNGGRTTRWANA-3′
DIAPLMF1	5′-AAGCGTATTYAYTTAYACCRACASCAACAA-3′
DIAPLMF2	5′-AGTGTCATCAACCAGWTCTYBGGSTAYTTC-3′
DIAPLMR1	5′-CATCCTBGACACYTCCYTCTCVGCCTTCTG-3′
DIAPLMR2	5′-CATCATCCACTTTYTTSCCRAASAGCTGCA-3′
DIAPS1F1	5′-TCCCATGTCCGGAGAVGAVGABTTYTACCT-3′
DIAPS1F2	5′-GGCCTTCGARCGHTACATYGTYATCTGCAA-3′
DIAPS1R1	5′-CACCACSACCATSCGVGASACCTCCCGCTC-3′
DIAPS1R2	5′-TTAGCTGGGGCYGACYTGRCTGGAGGACAC-3′
DIAPR1F1	5′-GTCAAAATTTCTAYRTBCCCWTKTCCAACA-3′
DIAPR1F2	5′-AATAGGATGCWRCWTYGARGGCTTCTTTGC-3′
DIAPR1R1	5′-ACAGTGCAGACAAGRYKYCCRTAGCAGAAG-3′
DIAPR1R2	5′-ATTCTTTCCACARCARAGRGTBRTGATCAT-3′
PESBLWSF	5′-GCGCGAATTCCACCATGCAAACAAGGGGGAAAGAA-3′
PESBLWSR	5′-CGGCGTCGACGCTGCTGGCGATACAGAAGAGTT-3′
PESBSWS1F	5′-GCGCGAATTCCACCATGTCCGGAGAAGAGGACTTC-3′
PESBSWS1R	5′-CGGCGTCGACGCGCTCCTTGGAGAACCCGTGGT-3′
PESBRH1F	5′-GCGCGAATTCCACCATGAATGGAACAGAAGGGCTT-3′
PESBRH1R	5′- CGGCGTCGACGCGGCAGGGGAAACCTGACTTGT-3′