Figure 5.
PSD-95 expression is mTOR dependent. A, B, Hippocampal slices were either not treated (control) or treated with 1.5 μm insulin. A, Insulin activates the mTOR. The graph presents cumulative data (n = 5; p < 0.02). The top panel depicts representative immunoblots of phospho-mTOR and total mTOR. B, Insulin treatment upregulates the expression of PSD-95. The graph represents cumulative data (n = 5; p < 0.02). The top panel depicts representative immunoblots of PSD-95 and actin. C, The expression of PSD-95 in vivo is mTOR dependent. Within 45 min of local rapamycin application to the GC, the levels of PSD-95 are significantly reduced (n = 5; p < 0.02).
