Figure 1.

Glioblastoma multiforme tumors express BMI1 and EZH2. A, Formalin-fixed paraffin-embedded GBM samples were immunolabeled with antibodies against BMI1 or EZH2 (brown) and GFAP or Nestin (pink). Arrowheads point to double positive cells. B, Frozen GBM samples were immunolabeled with antibodies against BMI1 (red) and CD133 (green). DAPI (blue) stains nuclei. Arrowheads point to double positive cells. C, RT-PCR analysis performed on a freshly isolated GBM sample with primers against GAPDH, BMI1, MUSASHI, SOX2, and LHX2. D, Frozen GBM samples were immunolabeled with antibodies against BMI1 (red) and CD31 (green). DAPI (blue) stains nuclei. Arrowheads point to BMI1 cells surrounding a blood vessel. Dashed lines (in A and B) delineate the border of the tumor. Scale bars, 100 μm.