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. 2009 May 13;29(19):6114–6123. doi: 10.1523/JNEUROSCI.0275-09.2009

Figure 3.

Figure 3.

Analysis of Ryr expression in the SCN and Ryr promoter activity in vitro. a, Circadian rhythm of Ryr1 and Ryr2 mRNA levels in the SCN of BMAL1+/+ and BMAL1−/− mice determined by real-time PCR. Data points represent mean ± SEM (n = 4) relative to highest Ryr2 mRNA levels. One-way ANOVA revealed circadian rhythms only in BMAL1+/+ mice. One-way ANOVA, *p < 0.05, **p < 0.01 versus CT14. b, Location of E-box-like elements and sequences of the 5′ flanking regions in the Ryr genes used for luciferase reporter assay. Truncated versions of the Ryr2 promoter are indicated with Mut1–3. c, Transcriptional activation of the luciferase reporter without (pGL4.10) or with the full-length Ryr promoter region (pGL4.10Ryr2) or with the first truncated promoter region (Ryr2Mut1) and in the presence and absence of CLOCK, BMAL1, and CRY1 expression vectors. Bars represent mean ± SEM of three independent experiments (§p < 0.01 vs the first bar; ***p < 0.01 vs the second bar; +++p < 0.001 vs the third bar; $p < 0.01 vs the second bar; ###p < 0.01 vs the third bar).