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. 2009 May 13;29(19):6068–6077. doi: 10.1523/JNEUROSCI.5597-08.2009

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Copyright © 2009 Society for Neuroscience 0270-6474/09/296068-10$15.00/0

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Figure 3. — Increase in cholesterol accompanies psychosine accumulation in TWI lipid rafts. A, Fluorometric analysis of total cholesterol concentrations are expressed in microgram per gram of tissue and show no change between WT and TWI at P3, P20, or P40. B, Assay of esterified cholesterol as a percentage of the total shows that less esterified cholesterol is found in TWI brains when compared with WT at P3, P20, and P40. C, Levels of cholesterol in raft fractions are expressed as a percentage of the total. This shows a preferential distribution of cholesterol concentrations in LR samples (fractions 4–5). D, Total levels of cholesterol in LRs (fractions 4–5) are increased in a manner that may be correlated to decreases in esterified cholesterol. These measurements are also conveyed as a percentage of the total. Data are expressed as a mean ± SE from two to four mice per time point.