Figure 4.

Redistribution of raft marker proteins accompanies psychosine accumulation in TWI lipid rafts. Lipid raft fractions from wild-type (W) and twitcher (T) nervous tissue were subjected to Western blot analysis. Representative blots are presented and show distributions of both raft Flotillin-2 (Flot 2)- and Caveolin-1 (Cav 1)- and nonraft (P115)-markers. These blots show that raft-marker distribution in the twitcher at all time points and in all tissues is disrupted in conjunction with the accumulation of psychosine. Proteins were also analyzed in total homogenates (To) and pellets (Pe). Actin was used as a house-keeping gene in Pe and To fractions.