Figure 1.

Association of huntingtin with PSD-95 and SAP102. A, PSD-95 associates with htt in YAC transgenic mouse striatal tissues. Lysates from 4- to 8-week-old mouse striatal tissue were immunoprecipitated with MAB2166 or HD650 (an anti-human htt-specific antibody). The blot was cut into two parts along the 160 kDa marker and probed with anti-htt and anti-PSD-95 antibodies, respectively. The mean band density ratio of PSD-95 to huntingtin for pooled data is shown below (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). Significant by one-way ANOVA and Bonferroni's post hoc tests, *p < 0.05 (n = 3). B, SAP102 associates with huntingtin in YAC transgenic mouse striatal tissue. Lysates from 4-week-old YAC transgenic mouse striatal tissue were immunoprecipitated with MAB2166 or HD650. The blot was cut into two parts along the 160 kDa marker and probed with anti-htt and anti-SAP102 antibodies, respectively. Pooled data for SAP102/htt density ratios are shown below representative blot. Ratios are similar across all three genotypes (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). p > 0.05 by one-way ANOVA; n = 7 for YAC18 and YAC72, n = 5 for YAC128. C, SAP102 associates with huntingtin in HEK293T cells. Lysates from HEK239T cells transfected with full-length htt15 or htt138, and SAP102 were immunoprecipitated and probed as described in B. Note that SAP102/htt density ratios are similar for htt15 and htt138.