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. 2009 Sep 2;29(35):10928–10938. doi: 10.1523/JNEUROSCI.2491-09.2009

Figure 2.

Figure 2.

Effect of mutant htt expression on interaction of NR2B with PSD-95 and SAP102. A, Mutant htt expression alters the association of PSD-95 with NR2B in vivo. NR2B was immunoprecipitated from lysates of 8-week-old WT and YAC transgenic mouse striatal tissue using an anti-NR2B antibody (Affinity BioReagents), and proteins were subjected to Western blot analysis. The blot was cut into two parts along the 160 kDa marker and probed with anti-NR2B and anti-PSD-95 antibodies, respectively. The mean band density ratio of PSD-95 to NR2B for pooled data is shown below (in each experiment, YAC72 and/or YAC128 were run on same gel as WT, and band density ratios were normalized to those in WT lane). Significant by one-way ANOVA and Bonferroni's post hoc tests, *p < 0.05, ***p < 0.001 (n = 8 for WT; n = 7 for YAC72 and YAC128). B, Representative blot showing that coimmunoprecipitation of SAP102 with NR2B in 4-week-old YAC mouse striatal tissue is not altered by htt repeat length. Pooled data for the mean band density ratio of SAP102 to NR2B is shown (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). There is no significant difference by one-way ANOVA, p > 0.05 (n = 6 for YAC18 and YAC128; n = 5 for YAC72). C, Representative blot showing that coimmunoprecipitation of PSD-95 with NR2A in 8-week-old YAC mouse striatal tissue is not altered by htt polyQ repeat length. Pooled data for the mean band density ratio of PSD-95 to NR2A is shown. There is no significant difference by one-way ANOVA, p > 0.05 (n = 3 for YAC18; n = 5 for WT and YAC72; n = 4 for YAC128).