Figure 2.

Selective deletion of GluN2B subunit in CA3 pyramidal cells of CA3-GluN2B-KO mice. Immunohistochemistry for GluN2B in mutant and control mice at 2 months of age. Immunoreactivities for GluN2B in the whole brain (A, B), hippocampus (E, F), and the dorsal and ventral hippocampi (G, H). Normal histology of the hippocampus in mutant mice (C, D). Immunoreactivities for calbindin used as a marker for subfields of the hippocampal formation (I, J): dense somal and dendritic labeling in the dentate gyrus (DG), dense mossy fiber labeling in the CA3 (CA3), and low diffuse labeling in the CA1. The arrows in G–J indicate the CA3 subfield. CB, Calbindin; dH, dorsal hippocampus; vH, ventral hippocampus. Scale bars: A, B, 1 mm; C–J, 0.1 mm.