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. 2009 Jul 22;29(29):9244–9254. doi: 10.1523/JNEUROSCI.1532-09.2009

Figure 7.

Figure 7.

ATP production deficiency in Afg3l2+/Emv66 mice. a, ATP synthesis rates in mitochondria isolated from cerebellum at 4, 6, and 12 months. Basal, No substrates (basal activity); PM, pyruvate and l-malate (complexes I–V); GM, glutamate and l-malate (complexes I–V); SR, rotenone and succinate (complexes II–V); AT, ascorbate and TMPD (complexes IV and V). Afg3l2+/Emv66 mice show impairment of ATP production starting at 6 months of age. Student's t test, *p < 0.05, **p < 0.001; n = 3. b, BN-PAGE–immunoblot analysis of complex I revealed by an anti-39 kDa antibody and immunoblot analysis of complex III revealed by anti-core2 (25.6 kDa subunit) antibody at 6 and 12 months; immunoblotting with anti-HSP60 antibody was used to verify equal loading. The graph shows protein amount of complexes I and III were quantified by densitometric analysis. Student's t test, *p < 0.05; n = 3. Error bars represent ±SD.