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. 2009 Jul 22;29(29):9244–9254. doi: 10.1523/JNEUROSCI.1532-09.2009

Figure 8.

Figure 8.

Increased level of oxidized proteins in mutant PCs. a, Total lysates and mitochondrial enrichments from cerebellum tissue from Afg3l2+/Emv66 and syngenic control mice were treated with DNPH. Derivatized proteins were analyzed by SDS-PAGE using an antibody against DNP moiety. Silver staining was used to verify equal loading. The untreated sample (treated with TFA only) was used as the negative control. M, Mitochondrial enrichment; TL, total lysate. b, Immunohistochemical localization of protein carbonyls in the Afg3l2+/Emv66 mouse cerebellum at 12 months of age. PC bodies are heavily stained in the Afg3l2+/Emv66 cerebellum (arrows). Scale bar: top, 100 μm; bottom, 50 μm. The graphs represent the count of DNP+ cells over the total cell number in mutants versus controls. Error bars represent ±SD; Student's t test, *p < 0.05; n = 2.