Figure 2.

Synchronized oscillations in MLI spiking and Ca²⁺ rises induced by mGluR1 activation. A, ΔF/F_o signals from the soma of a preloaded MLI (top trace) were acquired in parallel with the membrane currents recorded by a patch pipette in the cell-attached configuration (bottom trace). This MLI showed no spiking activity at rest; bath application of 2 μm quisqualate induced spike bursts and oscillatory changes in Ca²⁺. The gap in the current record corresponds to a short pause in the acquisition of the electrophysiological signal. B, Analysis of the ΔF/F_o signals and of the instantaneous action potential frequency from another MLI during the first (B1) and second (B2) transients of an oscillatory response to bath applied quisqualate. Note that the first transient exhibits less adaptation for both, the Ca²⁺ transient and the spike frequency increase.