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. 2009 Jul 22;29(29):9380–9389. doi: 10.1523/JNEUROSCI.0604-09.2009

Figure 5.

Figure 5.

YFP expression in the adult telencephalon of Nkx5-1-Cre;Rosa26R-YFP mice. A, B, Images of a coronal section through the telencephalon of P30 Nkx5-1-Cre;Rosa26R-YFP mice showing the distribution of YFP-expressing cells. B, High-magnification image of the boxed area shown in A, in which the overall laminar distribution of YFP-expressing cells is visualized. Note that whereas YFP-expressing cells are abundant in medial regions of the preoptic area, very few cells are found in the region of the magnocellular preoptic nucleus (MCPO). C, Many YFP-expressing cells in the neocortex of a P30 Nkx5-1-Cre;Rosa26R-YFP mice have the morphological features of cortical interneurons. D–D″, Immunohistochemistry against YFP (D, D″) and NeuN (D′, D″) in the neocortex of P30 Nkx5-1-Cre;Rosa26R-YFP mice. The arrowheads indicate YFP-expressing cells that express NeuN, whereas the open arrowhead points to a YFP-expressing cell that does not contain NeuN. E, Quantification of the distribution of E12.5 (light gray bars)- and E15.5 (dark gray bars)-born YFP-expressing cells in the neocortex of P30 Nkx5-1-Cre;Rosa26R-YFP mice (n = 3). ***p < 0.001 and **p < 0.01, t test. Error bars indicate SEM. Cg, Cingulate cortex; CPu, caudoputamen nucleus; I–VI, cortical layers I to VI; ICx, insular cortex; Ls, lateral septum; MCx, motor cortex; MPOA, medial preoptic area; Ms, medial septum; SCx, somatosensory cortex. Scale bars: A, 500 μm; B, 250 μm; C, D–D″, 50 μm.