Table 1.

Summary of MARCM analysis with Vglut–Gal4 of leg motor neurons^a

Lineage (embryonic clones)	Total # of MNs	# of times entire lineage was observed (# embryonic clones)	Latest time entire lineage was labeled (hours AEL)	Cell body position relative to neuropil	Non-MNs in the clone?	Partial lineages observed?	Targeted leg segments (#)	Notes
Ab	28c	69 (16)	≤48	Anterior	No	Yes	Fe (13), Ti (15)	9 of 10 embryonic clones also labeled F or G; may correspond to NB 15 of Truman et al. (2004)
B	7	47 (11)	≤54	Anterior	No	Yes	Fe (1), Tr (2), Co (4)
C	1	18 (2)	≤54	Posterior	No	NA	Co
D	2	77 (8)	≤66	Anterior	No	Yes	Co (2)
E	1	54 (10)	≤48	Posterior	∼25 inter-neurons	NA	Co
Fb	1	12 (5)	≤78	Anterior	No	NA	Co	Some single-cell clones project to coxa and femur
Gb	1	32 (5)	≤64	Anterior	No	NA	Tr
H	2	2 (1)	≤24	Anterior	No	NA	Fe (2 )	Embryonic born MN
I	2	1 (1)	≤12	Anterior	No	NA	Fe (2 )	Embryonic born MN
J	1	1 (1)	≤12	Posterior	No	NA	Co	Embryonic born MN
K	1	1 (1)	≤12	Anterior	No	NA	Fe	Embryonic born MN
Other MNs (only postembryonic labeling)
V	1	7 (0)	≤78	Anterior	No	NA	Fe
W	1	2 (0)	≤76	Anterior	No	NA	Fe
X	1	2 (0)	≤86	Anterior	No	NA	Fe
Y	1	2 (0)	≤78	Anterior	No	NA	Fe

^aTwo Vglut–Gal4⁺ motor neurons (MNs) were never observed in the MARCM analyses (one in coxa, one in trochanter). For those lineages that were only observed once (I–K), we cannot rule out that these neurons are part of another lineage.

^bFor Lin A, F, and G, the entire lineage can be labeled by both embryonic and postembryonic clones without labeling any additional motor neuronss, arguing that all three are independent lineages.

^cTwenty-seven of the 28 Lin A motor neurons were observed as single-cell clones. The single unlabeled Lin A motor neuron has a very distinctive axon targeting the ventral tibia.