Figure 3.

Effects of pharmacological manipulation of GABAergic transmission on fGDPs in WT and NKCC1^−/− slices. A, Isoguvacine (isog) at 5 μm induces a pronounced transient increase in fGDP frequency in P0–P1 WT but not in P0–P1 NKCC1^−/− slices. Mean ± SEM of fGDP frequency in seven WT and six NKCC1^−/− slices during isoguvacine application. B, Left, fGDPs are blocked by bumetanide (bume) in WT but not in NKCC1^−/− slices (bandpass, 0.3–20 Hz). Right, Mean ± SEM of normalized fGDP frequency obtained from six WT and six NKCC1^−/− slices and the effect of 10 μm bumetanide. C, fGDPs in WT slices reappear during a prolonged application of bumetanide.