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. 2009 May 27;29(21):7079–7091. doi: 10.1523/JNEUROSCI.0957-09.2009

Figure 3.

Figure 3.

Differentiation of transplanted interneuron progenitors. a, b document the distribution of BrdU-immunoreactive cells dissected from P7 β-actin-GFP donor rats, 1, 2, or 4 d after transplantation to P1 syngenic hosts (11–106 cells/case, 3 cases/time point). BrdU was injected 6 h before killing. The vast majority of donor cells proliferate in the PWM (arrows in b). c–j shows the progressive integration of P7 cerebellar progenitors derived from Pax- 2GFP/β-actin RFP mice transplanted to P1 mouse cerebella killed 4 d after graft. Young postmitotic interneurons, highlighted by increasing expression of the GFP reporter, initially appear in the PWM (c, d) and move to the cortex (e), where they progressively acquire mature phenotypic traits. f–h, Typical morphogenic phases of molecular layer (ML) interneurons, which first migrate to the edge of the external granular layer (f) and then develop characteristic neurites (g, h). i, j illustrate the distribution of the whole population of RFP-positive grafted cells (i) or of the Pax-2/GFP interneurons (j) at 1, 2, and 4 d after transplantation: note the progressive shift of donor interneurons from the PWM to the cortex. Survival times: b, 1 day post-transplantation (dpt); c–h, 4 dpt. GL, Granular layer. Scale bars: (b, g, h), 20 μm; (c, e), 50 μm; (d, f), 10 μm; (e–k,o), 20 μm.