Figure 6.

Nb+/VAChT−/GAD− Off cell. A, Nb-labeled cell (filled arrowhead, #ABS54) was negatively immunostained for VAChT (open arrowhead) and for GAD (open arrowhead), while located near other VAChT+ and GAD+ cells in the region (small arrows). Scale bar, 20 μm. The cell was situated in the MPPT (largest green open square) (Fig. 1, A0.9). B, C, The unit discharged at a fast average rate (13.28 Hz) in association with irregular slow-wave activity on the EEG of the retrosplenial cortex (RS Cx) before stimulation (Pre). It decreased its average discharge rate (to 4.20 Hz) in association with faster cortical activity during somatic stimulation. After stimulation (Post), the unit recovered its baseline rate in association with irregular slow-wave activity. Traces in B (enclosed by dashed lines) are expanded in C (arrows). The unit had a medium duration spike (shown in C, 0.49 and 1.34 ms at first and second zero crossings).