Figure 3.

PC12 cells express TN-C. A, Western blot analysis of cell extracts (left panels) and conditioned media (right panels) from differentiated PC12 cells transfected with the human integrins α4, α5, α5/α9, α9/α9, α9/α4, α9/α5, or α9 lacking the entire cytoplasmic domain (α9*) cultured for 48 h on PDL and examined for expression of osteopontin and full-length TN-C. Western blots were probed with rabbit IgG as a control. B, Full-length TN-C immunostaining in differentiated PC12 cells. Scale bar, 50 μm.