Figure 2.

Mo-mediated downregulation of reggie in RGCs leads to a reduction of regenerating axons. Mos were applied to the ZF optic nerves as described. Alexa-488–dextran was inserted into a second lesion site 2–3 mm distal from the first one at 7 d (A, B, C), 10 d (D, E, F), and 14 d (G, H, I), respectively. Counts of retrogradely labeled RGCs show that their number (A, D, G) was reduced in the reggie Mo-receiving RGCs (B, E, H) compared with the control Mo-containing RGCs (C, F, I): by 69% at 7 d, by 53% at 10 d, and by 33% at 14 d after ONS. Scale bars, 50 μm.