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. 2009 May 20;29(20):6607–6615. doi: 10.1523/JNEUROSCI.0870-09.2009

Figure 6.

Figure 6.

Coimmunoprecipitation assays for the analysis of the complex of Arp3, N-WASP, and cortactin and phosphorylation of cofilin and cortactin in reggie siRNA-treated N2a cells. A, In IGF-1 stimulated control cells, Abs against N-WASP and cortactin coprecipitate Arp3. In reggie-1 siRNA-treated cells, the Arp3, N-WASP, cortactin complex is present at lower levels and is almost disrupted during IGF stimulation. B, The phosphorylation of cortactin at Tyr466 is unchanged after IGF-1 treatment of control cells but is strongly reduced during IGF-1 treatment of reggie-1 siRNA cells. C, The phosphorylation of cofilin at Ser3 slightly decreases during IGF stimulation but is increased in reggie-1 siRNA-treated cells with or without IGF-1.