Figure 5.

D₂R-mediated signaling is altered in the absence of NCAM. A, Schematic representation of signaling pathways mediated by presynaptic short isoform of D₂R (D₂S) and postsynaptic long isoform of D₂R (D₂L). The activation of D₂S and D₂L leads to the inhibition of phosphorylation in TH and DARPP32, respectively. B, C, The levels of phosphorylated DARPP32 (pDARPP32) (B) and phosphorylated TH (pTH) (C) were analyzed by Western blot and normalized to total DARPP32 and TH levels in brain homogenates of adult NCAM^+/+ and NCAM^−/− mice.