Figure 5.

Local application of ATP into the rVRG/preBötC evokes a frequency response that closely follows the ATP concentration profile. Plots show, in a single medullary slice preparation, the effects of locally applying ATP within the rVRG/preBötC (0.1 mm, 10 s, starting at t = 0) on inspiratory frequency measured from integrated XII nerve recording (black traces) and the ATP difference current (ATP sensor current − null sensor current) within the rVRG/preBötC (gray traces). Consecutive responses to ATP applied at the same site, 15 min apart (left and middle). Inspiratory frequency and ATP difference current recorded in response to locally applying ATP at a site 140 μm medial to the initial site in the rVRG/preBötC (0.1 mm, 10 s; right).