Figure 3.

Immunocytochemical identification of juxtacellularly labeled MS neurons. Columns from left, Neurobiotin-labeled soma and/or dendrites; HCN1 immunoreactivity; PV immunoreactivity; light micrograph after nickel-intensified DAB reaction. A, The white arrows point to a medial septal neuron expressing both HCN1 and PV. B, Representative example of an MS neuron containing HCN1 but not PV (the immunoreactivity near the arrowhead belongs to a cell out of focus). PV-immunoreactive elements can also be observed in the vicinity of the labeled cell. n.b., Neurobiotin; l.m., light micrograph. Scale bars, 10 μm.