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. 2009 Dec 9;29(49):15542–15550. doi: 10.1523/JNEUROSCI.3938-09.2009

Figure 3.

Figure 3.

NFIL3 knockdown increases neurite outgrowth from primary adult DRG neurons. A, siRNA-mediated knockdown of NFIL3 protein levels was analyzed in HEK293 cells. Western blotting confirmed that siNFIL3 causes knockdown of NFIL3 protein in HEK293 cells overexpressing rat NFIL3. The siNFIL3 pool and two individual siRNAs (#2 and #3) significantly reduced NFIL3 protein levels; control siRNAs (siGLO and siCONTROL) did not affect NFIL3 protein levels. B, qPCR measurements confirm a 50–60% knockdown of Nfil3 mRNA levels in siNFIL3-transfected DRG neurons. C–E, Primary adult DRG neurons transfected with the siNFIL3 pool show a 51% increase in neurite length. Three of the four individual NFIL3 siRNAs (#2, #3 and #4) also induce a significant increase in neurite outgrowth. The length of the longest neurite was measured for 100–150 cells per condition. Note that the individual siRNAs resulting in the strongest increase in neurite length (E) also give the highest reduction in protein expression in HEK293 cells (A). Bars represent means ± SEM; *p < 0.01. Scale bar, 100 μm.