FasL–Fas interactions contribute to neither alteration of electrical properties of neurons nor neuronal killing by OT-I T cells. A, Cell death after 6 h of OT-I neuron coculture under control conditions (con; n = 16) and in the presence of a neutralizing anti-FasL antibody (10 μg/ml, n = 16; 50 μg/ml, n = 8) and an isotypic control (50 μg/ml, n = 6) as assessed by DAPI/PI staining. B, RM of hippocampal neurons after interaction with OT-I cells in the absence and presence of a neutralizing anti-FasL antibody (10 μm/ml, n = 8; 50 μm/ml, n = 8) and an isotypic control (Iso; 50 μm/ml, n = 6). Error bars represent mean ± SEM. **p < 0.05; ns, not significant.